BioReady Covalent Conjugate Optimization Kit
Gold Nanoshells are ultra-bright labels for lateral flow – providing up to 20× increases in sensitivity compared to 40 nm gold.
The BioReady Covalent Conjugate Optimization Kit provides everything you need* to get started optimizing your conjugation to Gold Nanoshells:
- Detailed protocol walks through the recommended first 4 experimental sweeps when optimizing a new conjugation
- Kit includes verified reagents & protocols including gold nanoshells, antibody purification filter, single-use EDC & NHS for 3 conjugation sweeps, reaction buffers, block buffer, and more
Gold nanoshell particles have a 120 nm silica core with a 15 nm gold shell; functionalized with a carboxyl (–COOH) surface and ready for covalent conjugation. Provided at 20 OD in 0.02 potassium carbonate solution.
* See product insert for list of materials and instruments needed but not provided.
Which Surface Should I Choose?
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BioReady carboxyl (–COOH) gold is an effective and economical nanoparticle for covalent conjugations to proteins through carbodiimide crosslinker chemistry. Covalent coupling of proteins (e.g. antibodies) to a gold nanoparticle surface yields robust and stable gold particle conjugates. The nanoparticles are surface functionalized with a tightly bound monolayer that contains terminal carboxylic acid functional groups which can be activated through EDC/Sulfo-NHS chemistry to generate gold nanoparticle-antibody amide bonds. The EDC/Sulfo-NHS chemistry requires an extra step to activate the carboxyl group but can be performed at scale at reasonable cost, making carboxyl gold the most cost-effective method of covalently binding antibodies to gold.
Certificate of Analysis Examples
Please note that these are representative Certificates of Analyses (CoAs) provided as examples for this product. We provide a unique batch-specific CoA with each product during shipment; only the CoA that arrives with your product should be referred to for actual characterization and measurement data. If you would like an electronic copy of the CoA for the product you received or the material(s) we currently have in stock, please contact us.
|Product Line||Surface||Example Certificate of Analysis||Product #||Price|
Gold nanoshells produced under license to US patent numbers 6,344,272, 6,685,986, and 7,371,457. Please see our Terms & Conditions for more information.